Journal: Current Research in Parasitology & Vector-borne Diseases
Article Title: Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania ( Mundinia ) martiniquensis and Leishmania ( Mundinia ) orientalis , responsible for autochthonous leishmaniasis in Thailand
doi: 10.1016/j.crpvbd.2024.100217
Figure Lengend Snippet: A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B ClustalW alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .
Article Snippet: Representative sequences of each locus from Leishmania spp. were aligned using ClustalW implemented in BioEdit version 7.2.6 ( ) to reveal the regions of high intraspecific conservation, which allowed us to design custom primers and probes for the duplex qPCR assay, as illustrated in , .
Techniques: Sequencing, Amplification