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Bioedit Company clustalw implemented in bioedit v7.0
Clustalw Implemented In Bioedit V7.0, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B <t>ClustalW</t> alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .
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A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B <t>ClustalW</t> alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .
Clustalw Algorithm Implemented In Bioedit V.7.0, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B ClustalW alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .

Journal: Current Research in Parasitology & Vector-borne Diseases

Article Title: Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania ( Mundinia ) martiniquensis and Leishmania ( Mundinia ) orientalis , responsible for autochthonous leishmaniasis in Thailand

doi: 10.1016/j.crpvbd.2024.100217

Figure Lengend Snippet: A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B ClustalW alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .

Article Snippet: Representative sequences of each locus from Leishmania spp. were aligned using ClustalW implemented in BioEdit version 7.2.6 ( ) to reveal the regions of high intraspecific conservation, which allowed us to design custom primers and probes for the duplex qPCR assay, as illustrated in , .

Techniques: Sequencing, Amplification

A Schematic representation of the intergenic region between two Leishmania HSP70-I loci. B ClustalW alignment of the HSP70-I IR revealed the sequence region used to design primers and a probe for amplification of L. orientalis with a product size of 64 bp. Note that the closely related L. chancei could potentially be amplified using primers and a probe designed for L. orientalis in this study.

Journal: Current Research in Parasitology & Vector-borne Diseases

Article Title: Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania ( Mundinia ) martiniquensis and Leishmania ( Mundinia ) orientalis , responsible for autochthonous leishmaniasis in Thailand

doi: 10.1016/j.crpvbd.2024.100217

Figure Lengend Snippet: A Schematic representation of the intergenic region between two Leishmania HSP70-I loci. B ClustalW alignment of the HSP70-I IR revealed the sequence region used to design primers and a probe for amplification of L. orientalis with a product size of 64 bp. Note that the closely related L. chancei could potentially be amplified using primers and a probe designed for L. orientalis in this study.

Article Snippet: Representative sequences of each locus from Leishmania spp. were aligned using ClustalW implemented in BioEdit version 7.2.6 ( ) to reveal the regions of high intraspecific conservation, which allowed us to design custom primers and probes for the duplex qPCR assay, as illustrated in , .

Techniques: Sequencing, Amplification